St. John's Research Institute

Post                                    : Project Associate II

Qualification                    : Qualified GATE/NET or Masters in Natural or Agricultural Sciences

No. of Vacancy                :  One

Language                         : English, Kannada,

Location                           : Bangalore, Karnataka

Application due date      : 22^nd December, 2021

Salary                               : Rs. 35,000+24%HRA

Brief Description about the Project:

 IMPORTANCE Proposal Hypothesis: Variation in innate immune activation and attendant cardiac damage and dysfunction collectively drive systemic immunopathology and account for the differences in COVID-19 mortality between SAs in the UK and India. Specific Aims to Address this Hypothesis 1. Assess the differences in pre-existing health conditions, disease severity and the pattern of in-hospital complications between SAs admitted with COVID-19 in the UK versus India to determine the contribution of pre-existing CVD/diabetes and cardiac dysfunction. Approach: Retrospective analyses of existing clinical and imaging datasets in both countries. 2. Determine whether there are inherent differences in microbially-induced proinflammatory and antiviral immune responses between SAs in UK versus India, and their association with CVD and autoantibodies to IFN. Approach; Study of non-infected subjects with a range of CV risk factors. 3. Perform extensive systemic immunophenotyping, and analysis of endothelial, complement activation, and cardiac function in SAs admitted with severe COVID-19 in London and India to identify key immune patterns / signatures associated with clinical outcome. Approach: Prospective study in patients hospitalised with severe COVID-19. 4. Determine the inter-relationship between immune responses, pre-existing CVD and functional outcome in SAs who have recovered from COVID19 in the UK versus India. Approach: Add-on analyses in recovered COVID-19 patients being monitored at 6-9 months post-discharge.

Key Skills

Advanced theoretical understanding of immunology at the Masters level The ability to set up and data interpret in the context of the field of research (infection and immunity: human immunology) a number of immune assays with initial supervision of assay set up including: advanced multiparameter flow cytometry; immune transcriptome analysis; immune subset purification and analysis; extensive experience in immune cell culture and stimulation; evidence of an in-depth understanding of T cell immunity to infection including knowledge of T cell subset function; cell sorting and capture for transcriptome analysis; qPCR and ability to set up cell migration and immune co-culture assays.

Ability to work independently on assay set up and be collegiate within a wider team of immunologists.

Ability to communicate experimental and demonstrate ability to learn how to interpret immune data

Undertake scholarly activity to review and interpret scientific literature and to present primary data using a variety of appropriate software

Willingness to undertake scientific administrative duties including ordering consumables and keeping financial and clinical records

Core Responsibilities:

• To conduct primary research as per the CARDINNATE award, including research towards stated goals and exploratory research within the confines of the BIRAC award in consultation with the PI
• To work under supervision of Lead Project Scientist of BIRAC project in set up of immune assays and processing of clinical samples.
• To undertake key scientific administrative duties in placing ordering and maintaining an overview of expenditure
• To communicate work plan and data in weekly scientific laboratory meetings
• To undertake scholarly research activities such as scientific literature analysis and to show keenness to keep abreast of the current field and to effectively communicate academic thinking

Specific Activities

• To isolate blood cells, archive and keep clinical records
• To set up under guidance and execute immune assays in conjunction with discussions with Project Scientist appointed to BIRAC project and with project In charge
• To place orders for consumables and maintain records of finance expenditure
• To effectively communicate with staff members of the wider department at SJRI and provide scientific data updates to the PI

To Apply                          : E-mail cover letter, CV, the names & contact details of Referees to yashashwini@sjri.res.in cc  hr@sjri.res.in

For more information please visit our website www.sjri.res.in

Can Contact us on the following Numbers 080-49467010/49467011