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Dr. Amit Kumar Mandal

  • Professor
  • amit@sjri.res.in
Education

  • 2005 - 2006, Post Doctoral Fellow - Structural Biology
    Molecular Biophysics Unit, IISc, Bangalore, Mentor: Prof. P Balaram (Director, IISc)
  • 2002 - 2003, Post Doctoral Fellow - Structural Biology
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, Mentor: Prof. Paul M Horowitz
  • 1994 - 2002, Ph.D. - Structural Biology
    Department of Biophysics, Bose Institute, Calcutta (degree awarded by Jadavpur University), Mentor: Prof. Siddhartha Roy (Present Director, IICB)
Experience

  • Professor
    St. John’s Research Institute
    May 2011 - till date
    Karnataka, India
  • Associate Professor
    St. John’s Research Institute
    Jan 2007 - April 2011
    Karnataka, India
  • Lecturer in Chemistry
    PB College
    July 2000 - Dec 2004
    West Bengal, India
Publications

Assessment of Cysteine Reactivity of Human Hemoglobin at Its Residue Level: A Mass Spectrometry-Based Approach. Mitra A, Muralidharan M, Srivastava D, Das R, Bhat V, Mandal AK. Hemoglobin. 2017; 1-6.

Suicidal behaviour is associated with decreased esterified cholesterol in plasma and membrane fluidity of platelets. Mathew B, Srinivasan K, Pradeep J, Thomas T, Mandal AK. Asian J Psychiatr. 2017;32:105-109.

Application of isotope exchange based mass spectrometry to understand the mechanism of inhibition of sickle hemoglobin polymerization upon oxygenation. Das R, Mitra A, Bhat V, Mandal AK. Journal of Structural Biology. 2017; 199(1):76-83.

Adsorption Induced Changes of Human Hemoglobin on Ferric Pyrophosphate Nanoparticle Surface Probed by Isotope Exchange Mass Spectrometry: An Implication on Structure-Function Correlation. Srinivasu BY, Bose B, Mitra G, Kurpad AV, Mandal AK. Langmuir. 2017;33(32):8032-8042.

Effect of ascorbic acid rich, micro-nutrient fortified supplement on the iron bioavailability of ferric pyrophosphate from a milk based beverage in Indian school children. Maria Pauline, Sejil T Verghese, Bindu Srinivasu, Beena Bose, Tinku Thomas, Amit Kumar Mandal, Prashanth Thankachan, Anura V Kurpad. Asia Pacific Journal of Clinical Nutrition, DOI: 10.6133/apjcn.092017.07.

Analysis of Isotopic Peaks in Hydrogen/Deuterium Exchange Mass Spectra for Precise Measurement of Isotope Average Centroid Mass of Peptides and Proteins Using R Programming Language. Arumugaperumal Arun, Sreekala Narayanan, Subhasis Sarangi, Amit Kumar Mandal. International Journal of Biochemistry Research & Review, 2017, 18:1-8.

Interaction of p-benzoquinone with hemoglobin in smoker’s bloodcauses alteration of structure and loss of oxygen binding capacity. Arunava Ghosh, Santanu Banerjee, Amrita Mitra, Monita Muralidharan,Bappaditya Roy, Rajat Banerjee, Amit Kumar Mandal, Indu B. Chatterjee. Toxicology Reports 3,2016 295-305.

Mass Spectrometry-Based Diagnosis of Hemoglobinopathies: A Potential Tool for the Screening of Genetic Disorder. Rajdeep Das, Gopa Mitra, Boby Mathew, Vijay Bhat, Cecil Ross, Debnath Pal, Amit Kumar Mandal. Biochemical Genetics 2016. 54:816–825.

Protein Structure-Function Correlation in Living Human Red Blood Cells Probed by Isotope Exchange-based Mass Spectrometry. Narayanan S, Mitra G, Muralidharan M, Mathew B, Mandal AK. Analytical Chemistrty. 2015, 87:11812-8.

Beneficiary effect of nanosizing Ferric Pyrophosphate as food fortificant in Iron Deficiency Anemia: evaluation of bioavailability, toxicity and plasma biomarker. Srinivasu B, Mitra G, Muralidharan M, Srivastava D, Pinto J, Thankachan P, Suresh S, Shet A, Rao S, Ravikumar G, Thomas T, Kurpad A, Mandal AK. Royal Society of Chemistry Advances. 2015, 5:61678-61687.

Mass spectrometry based characterization of Hb Beckman variant in a falsely elevated HbA1c sample. Das R, Muralidharan M, Mitra G, Bhat VS, Mathew B, Pal D, Ross C, Mandal AK. Analytical Biochemistrty. 2015, 489:53-8.

Characterization of a Double Heterozygote HbE/?(+) Thalassemia IVS 1-1 [G>T] in a Juvenile Diabetic. Bhat VS, Lokeshwarappa S, Mandal AK. Indian Journal of Clinical Biochemistry. 2015, 30:238-242.

Automated analysis of hemoglobin variants using NanoLC-MS and customized database. Das R, Mitra G, Mathew B, Ross C, Bhat V, Mandal AK. Journal of Proteome Research. 2013, 12(7): 3215-22.

Structural Proteomics Takes Aim at Disease: A combination of H/DX-MS and IMS will help to explore the basis of functional failures in proteins linked to medical conditions. Mitra A, Mandal AK. The Analytical Scientist. 2013, 0613: 303.

Glutathionylation induced structural changes in oxy human hemoglobin analyzed by backbone amide hydrogen/deuterium exchange and MALDI-mass spectrometry. Mitra G, Muralidharan M, Narayanan S, Pinto J, Srinivasan K, Mandal AK. Bioconjugate Chemistry. 2012, 23(12): 2344-53.

Glutathionyl Hemoglobin is elevated in Iron Deficiency Anemia. Shet AS, Pinto SM, Mitra G, Mandal AK. Acta Haematologica, 2011, 127: 26-30.

Co-inheritance of HbD (Iran)/Beta Thalassemia IVS1-5 (G>C) Trait in a Punjabi Lady with Diabetes. Bhat V, Mandal AK, Mathew B. Indian Journal of Clinical Biochemistry. 2012, 27(2): 202-6.

Identification of a Rare Hemoglobin Variant HbJ-Rajappen [alpha90 (FG2)Lys ? Thr] Using Mass Spectrometry. Bhat V, Mandal AK, Mathew B. Indian Journal of Clinical Biochemistry. 2012, 27(4): 414-6.

Structural perturbation of human hemoglobin on glutathionylation probed by hydrogen-deuterium exchange and MALDI mass spectrometry. Mitra G, Muralidharan M, Pinto J, Srinivasan K, Mandal AK. Bioconjugate Chemistry, 2011, 22: 785-793.

Analysis of hemoglobin variants using non-denaturing gel electrophoresis and matrix-assisted laser desorption ionization mass spectrometry. Mathew B, Bhat V, Mandal AK. Analytical Biochemistry, 2011, 416: 135-137.

Characterization of A Hemoglobin Variant: Hbq-India/Ivs 1-1[G >T]-?- Thalassemia. Bhat V, Dewan K, Krishnaswamy PR, Mandal AK, Balaram P. Indian Journal of Clinical Biochemistry, 2010, 25: 99-104.

Analysis of Protein Post-translational Modifications by Mass Spectrometry: With Special Reference to Hemoglobin. Woodi M, Mandal AK, Balaram P, Krishnaswamy PR. Indian Journal of Clinical Biochemistry, 2009, 24: 23-29.

Electrospray mass spectrometric characterization of hemoglobin Q (Hb Q-India) and a double mutant hemoglobin S/D in clinical samples. Mandal AK, Bisht S, Bhat V, Krishnaswamy PR, Balaram P. Clinical Biochemistry, 2008, 41: 75-81.

Sequencing of T-superfamily conotoxins from Conus virgo: Pyroglutamic acid identification and disulfide arrangement by MALDI mass spectrometry. Mandal AK, Ramasamy MS, Sabareesh V, Openshaw, Krishnan KS, Balaram P. Journal of the American Society for Mass Spectrometry. 2007, 18: 1396-1404.

Quantitation and characterization of glutathionyl hemoglobin as an oxidative stress marker in chronic renal failure by mass spectrometry. Mandal AK, Murali W, Varun S, Patanam RK, Anjali R, Sudarshan B, Padmanabhan B. Clinical Biochemistry. 2007, 40: 986-994.

Mass spectrometric identification of pyroglutamic acid in peptides following selective hydrolysis. Mandal AK, Padmanabhan B. Analytical Biochemistry, 2007, 370: 118-120.

Solvation dynamics of a protein in the pre-molten globule state. Samaddar S, Mandal AK, Mondal SK, Sahu K, Bhattacharyya K, Roy S. Journal of Physical Chemistry B. 2006; 110(42): 21210-5.

Glutamate counteracts the denaturing effect of urea through its effect on the denatured state. Mandal AK, Samaddar S, Banerjee R, Lahiri S, Bhattacharyya A, Roy S. Journal of Biological Chemistry. 2003; 278(38): 36077-84.

Solvation change and ion release during aminoacylation by aminoacyl-tRNA synthetases. Banerjee R, Mandal AK, Saha R, Guha S, Samaddar S, Bhattacharyya A, Roy S. Nucleic Acids Research. 2003; 31(20): 6035-42.

Solvation dynamics of a probe covalently bound to a protein and in an AOT microemulsion: 4-(N-bromoacetyl-amino)-phthalimide. Mandal D, Sen S, Sukul D, Bhattacharyya K, Mandal AK, Banerjee R, and Roy S. Journal of Physical Chemistry B 2002; 106: 10741-7.

Dynamics of compact denatured states of glutaminyl-tRNA synthetase probed by bis-ANS binding kinetics. Bhattacharyya A, Mandal AK, Banerjee R, Roy S. Biophysical Chemistry. 2000; 87(2-3): 201-12

A cognate tRNA specific conformational change in glutaminyl-tRNA synthetase and its implication for specificity. Mandal AK, Bhattacharyya A, Bhattacharyya S, Bhattacharyya T, Roy S. Protein Science. 1998; 7(4): 1046-51.

Application of Hydrogen/Deuterium Exchange Mass Spectrometry in Structural Biology and Molecular Medicine. Bindu Yadaganahalli Srinivasu, Amit Kumar Mandal. A chapter in the book "Advances in Biochemistry and Applications in Medicine”.

Proteomics in Hematology and Oncology: A Novel Tool for Clinical Investigation. Chapter in Hematology Today 2010 Edition. Arun S. Shet, Amit K. Mandal

Projects

Analysis of Hemoglobin Variants (In collaboration with IISC) - Principal Investigator - Funded by Department of Biotechnology, Govt. of India; Tenure: 2012-2017 - 1.4 crores.

Elucidation of the pathway of structural adaptation of tau from intrinsic disorder to highly structured filaments in Alzheimer’s disease: A mass spectrometry based approach. - Co-Principal Investigator - Funded by Department of Science and Technology, Govt. of India, Tenure: 2014-2017 - 50 lakhs.

Effect of nanoparticles on a biological system probed by quantification of biomarker, proteome profiling of tissues using Imaging Mass Spectrometry and conformational analysis of desorbed hemoglobin- - Principal Investigator - Funded by Department of Science and Technology, Govt. of India, Tenure: 2015-2018 - 3.5 crores.

Absolute quantification of plasma based biomarkers in patients with attempted suicide, in patients at high risk for attempting suicide and their first degree relatives using isotope labeled peptides - Principal Investigator - Funded by Department of Science and Technology, Govt. of India, Tenure: 2015-2018 - 50 lakhs.

Patents

"Low cost electrochemical disposable sensor for measuring glycated hemoglobin". Siva Rama Krishna Vanjari, Navakanta Bhat, Sampath Srinivasan, Bharadwaj Amrutur, Chakrapani Kalapu, Amit Kumar Mandal. United States Patent; Patent No: US 8,702,931 B2, Date of Patent: Apr 22, 2014.

Groups and Associations
Interests

Haemoglobin Disorder; Nano Particle Protein Interaction; Tissue Imaging; Psychiatric Disorder

Attachments

Dr. Amit Kumar Mandal